Phytase production by white-rot fungi cultivated in liquid medium with particulate agro-industrial byproducts

This study aimed to screen phytase-producing basidiomycetes by submerged cultivation (SmC) with particulate agro-industrial byproducts and evaluate the enzyme's thermal pH stability. Five among eight fungal species produced high phytase activity (> 300 U mL-1) after seven days in SmC. Ganoderma sp., best producer (1130 mL-1), was grown a liquid medium (coffee husks, citric pulp, sugarcane bagasse, soybean hulls). Soybean hulls increased (16%) productivity (2.3-fold). The residual determined 120 min incubation different (pH 3, 5, 7, 9) temperatures (30, 50, 70 oC). Phytase remained stable at ranging from 3 9, 100% retention 7. Furthermore, retained more than 60% when incubated for 30 oC oC. Our findings support future studies using sp. production potential use animal feed industry.